Summary
Type: SPE
Application #: 305300
Catalog#: 730739 - SPE Cartridges, Chromabond, HR-XCW, 3mL, 200mg, 30/pk
Detection:
Conditions: Column conditioning: 5 ml methanol, then 5 ml water
(Do not let run the column dry!)
Sample aspiration: The sample is passed through the column by vacuum or pressure (max. 1000 ml sample volume).
Washing 1: 2 ml acidified water
Washing 2 / Elution 1: 2 ml methanol (elution of neutral and acidic compounds)
Drying: With nitrogen or air
Elution 2: 2 x 2 ml 1 - 5 % formic acid in methanol (elution of strongly basic compounds)
(Basic methanol (NH3) can be used alternatively for elution 2 (e.g. for primary to tertiary amines). Here an interruption of the interactions with the cation exchanger results by a deprotonation of the analyte.)
Evaporation and reconstitution (if necessary)
Authors: SPE department
Source: Macherey-Nagel; Germanny 2009